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Product Info Summary
| SKU: | PA1659 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Lipocalin-1 LCN1 Antibody
View all Lipocalin-1 Antibodies
SKU/Catalog Number
PA1659
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Lipocalin-1 LCN1 Antibody catalog # PA1659. Tested in IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Lipocalin-1 LCN1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1659)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human LCN1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1659 is reactive to LCN1 in Human
Applications
PA1659 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
19 kDa
Calculated molecular weight
19.25kDa
Background of Lipocalin-1
Lipocalin-1 (LCN1), also known as PREALBUMIN, TEAR or VEGP, is a protein that in humans is encoded by the LCN1 gene. Lipocalin-1 is a member of the lipocalin family of small secretory proteins, which is mapped on 9q34.3. Lipocalin-1 is the primary lipid binding protein in tears and is overproduced in response to multiple stimuli including infection and stress. It may be a marker for chromosome aneuploidy as well as an autoantigen in Sjogren's syndrome. LCN1 inhibits the cysteine-protease papain in vitro, similar to cystatins. And LCN1 plays a role in the nonimmunologic defense and in the control of inflammatory processes in oral and ocular tissues. The investigations presented the first clear evidence that LCN1 is induced in infection or inflammation and supported the idea that this lipocalin functions as a physiologic protection factor of epithelia in vivo.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
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Anti-LCN1 antibody, PA1659, Western blotting
All lanes: Anti LCN1 (PA1659) at 0.5ug/ml
Lane 1: JURKAT Whole Cell Lysate at 40ug
Lane 2: COLO320 Whole Cell Lysate at 40ug
Lane 3: SCG Whole Cell Lysate at 40ug
Lane 4: HELA Whole Cell Lysate at 40ug
Predicted bind size: 19KD
Observed bind size: 19KD
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Anti-LCN1 antibody, PA1659,IHC(P)
IHC(P): Human Prostatic Cancer Tissue
Protein Target Info & Infographic
Gene/Protein Information For LCN1 (Source: Uniprot.org, NCBI)
Gene Name
LCN1
Full Name
Lipocalin-1
Weight
19.25kDa
Superfamily
calycin superfamily
Alternative Names
LCN1; lipocalin 1 (tear prealbumin); lipocalin 1-like 2; Lipocalin1; Lipocalin-1; MGC71975; PMFA; Tear lipocalin; Tear Prealbumin; TLC; TPVon Ebner gland protein; VEG protein; VEGP; VEGPlipocalin 1 (protein migrating faster than albumin, tear prealbumin) LCN1 PMFA, TLC, TP, VEGP lipocalin 1 lipocalin-1|VEG protein|Von Ebner gland protein|protein migrating faster than albumin|tear prealbumin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LCN1, check out the LCN1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LCN1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Lipocalin-1 LCN1 Antibody (PA1659)
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No publications found for PA1659
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Customer Reviews
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Customer Q&As
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1 Customer Q&As for Anti-Lipocalin-1 LCN1 Antibody
Question
We are currently using anti-LCN1 antibody PA1659 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2018-10-09
Answer
The anti-LCN1 antibody (PA1659) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-10-09
